Thermogenic Fat

This volume presents a broad collection of state-of-the-art methods to study the biology of thermogenic fat using in vitro cell culture and animal models. Chapters guide the readers through protocols on differentiation of human pluripotent stem cells and murine adipocyte precursors; methods for measuring mitochondrial respiration, heat generation, brown fat activation, and effects on energy metabolism in mice; and techniques for AAV-mediated gene delivery, transplantation of adipose tissue, isolation of adipose tissue immune cells and extracellular vesicles, and mass spectrometry-based profiling of brown fat lipids. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Thermogenic Fat: Methods and Protocols aims to be comprehensive guide for researchers in the field.

This eBook is a collection of articles from a Frontiers Research Topic. Frontiers Research Topics are very popular trademarks of the Frontiers Journals Series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area! Find out more on how to host your own Frontiers Research Topic or contribute to one as an author by contacting the Frontiers Editorial Office: frontiersin.org/about/contact.

Revolutionary research breakthrough that restores man's natural ability to burn unwanted fat.

Obesity has emerged as a major threat to public health in both the western and developing world. Essentially a disorder of energy balance, obesity occurs when energy intake and storage exceeds expenditure. Much of energy homeostasis depends on the activity and function of adipose tissue. Adipocytes in mammals fall into two categories classified by their primary functions: white fat cells that mediate energy storage and thermogenic fat cells that counteract hypothermia and obesity through adaptive thermogenesis. Whereas white fat and its function as an energy reservoir and endocrine organ have been studied for decades and are relatively well understood, until recently many aspects of the thermogenic fat biology have remained elusive. Accumulating evidence supports the hypothesis that thermogenic fat cells arise from at least two different developmental origins: the ones of a skeletal muscle-like lineage are now called “classical” brown fat cells, and the rest of the thermogenic fat cells are normally referred to as the beige fat cells. The last decade has witnessed an explosion of interest and studies focusing on the regulation of thermogenic fat cells and potential therapeutics targeting these adipocytes. Here we summarize the recent advancements in our understanding of these metabolically active fat cells.

Author of the popular Primal Body, Primal Mind and widely recognized Paleo expert and nutritionist Nora Gedgaudas presents a revolutionary and accessible high-fat diet for maintaining health, losing weight, and reversing some chronic diseases. Popular nutritionist Nora Gedgaudas returns with advice that may sound counterintuitive: Eat fat to burn fat. In Primal Fat Burner, she explains the benefits and science behind a ketogenic (or fat-burning) diet, which switches your metabolism from a dependence on sugar to running on healthy fats. As Gedgaudas reveals, numerous studies in recent years refute the long-promoted anti-saturated fat and anti-cholesterol agenda. Now, “with able, funny writing” (Booklist), Gedgaudas explains the science that fat isn’t always a bad thing—if you know the right kinds of fats to eat. In her comprehensible, enjoyable style, she also lays out a practical meal plan with recipes. Author of the bestselling Grain Brain, Dr. David Perlmutter writes in his foreword that Primal Fat Burner is, “Wonderfully actionable, compassionately taking the reader from ‘why’ to ‘how.’” On this diet, you efficiently and effectively metabolize fat (ketones and free fatty acids) as your primary source of fuel, rather than glucose from carbs, starches, and sugars. Because fat is so satisfying, you naturally wind up eating less—without feelings of hunger or deprivation. And natural dietary fat is ultimately the key to feeling better, looking better, thinking clearer, and living longer. Discover all this and more healthy tips in one of the most groundbreaking nutritional books in recent time.

A creator of the Fresh Start Metabolism Program shares her personal success story of overcoming morbid obesity as well as the inspirational stories of people she has helped, in a guide that identifies thermogenic foods, from hot peppers and apple cider vinegar to salsa and celery, that can help people to lose weight regardless of body type.

With guaranteed weight-loss of three kilos (seven lbs) a week without feeling hungry, Fat-burner Foods provides a safe and realistic way to diet successfully. The diet guarantees satisfactory weight reduction whilst also including all the essential food groups, simultaneously banning hunger and boosting energy levels. With a comprehensive four day eating plan and a range of easy-to-prepare, adaptable recipes, Fat-burner Foods provides a dietary plan that helps establish healthier eating and ensures the weight stays off.

Cathi Graham lost over 186 pounds and kept if off for more than 20 years with a simple diet secret. Now she's ready to share that secret with you. In 1982, Cathi Graham tipped the scales at 326 pounds and her doctor scribbled three words on her medical chart that would forever change her life: “patient morbidly obese.” Horrified, she began intensive research and discovered the power of thermogenic foods–foods that stimulate your metabolism and help you burn fat. Complete with inspirational stories from thousands of people who have lost weight on the diet, The Fresh Start Thermogenic Diet provides an eating plan to fit your specific needs and body type, including a special program to help end emotional eating. Thermogenic foods, such as salsa and green tea, help you lose weight no matter what your body type. A proven method for learning to love your body and yourself, The Fresh Start Thermogenic Diet is an easy and effective way for anyone who wants to take off anywhere from 8 to 186 pounds– and keep it off.

Obesity has emerged as a major threat to public health in both the western and developing world. Essentially a disorder of energy balance, obesity occurs when energy intake and storage exceeds expenditure. Much of energy homeostasis depends on the activity and function of adipose tissue. Adipocytes in mammals fall into two categories classified by their primary functions: white fat cells that mediate energy storage and thermogenic fat cells that counteract hypothermia and obesity through adaptive thermogenesis. Whereas white fat and its function as an energy reservoir and endocrine organ have been studied for decades and are relatively well understood, until recently many aspects of the thermogenic fat biology have remained elusive. Accumulating evidence supports the hypothesis that thermogenic fat cells arise from at least two different developmental origins: the ones of a skeletal muscle-like lineage are now called "classical" brown fat cells, and the rest of the thermogenic fat cells are normally referred to as the beige fat cells. The last decade has witnessed an explosion of interest and studies focusing on the regulation of thermogenic fat cells and potential therapeutics targeting these adipocytes. Here we summarize the recent advancements in our understanding of these metabolically active fat cells.

Author of the popular Primal Body, Primal Mind and widely recognized Paleo expert and nutritionist Nora Gedgaudas presents a revolutionary and accessible high-fat diet for maintaining health, losing weight, and reversing some chronic diseases. Popular nutritionist Nora Gedgaudas returns with advice that may sound counterintuitive: Eat fat to burn fat. In Primal Fat Burner, she explains the benefits and science behind a ketogenic (or fat-burning) diet, which switches your metabolism from a dependence on sugar to running on healthy fats. As Gedgaudas reveals, numerous studies in recent years refute the long-promoted anti-saturated fat and anti-cholesterol agenda. Now, “with able, funny writing” (Booklist), Gedgaudas explains the science that fat isn’t always a bad thing—if you know the right kinds of fats to eat. In her comprehensible, enjoyable style, she also lays out a practical meal plan with recipes. Author of the bestselling Grain Brain, Dr. David Perlmutter writes in his foreword that Primal Fat Burner is, “Wonderfully actionable, compassionately taking the reader from ‘why’ to ‘how.’” On this diet, you efficiently and effectively metabolize fat (ketones and free fatty acids) as your primary source of fuel, rather than glucose from carbs, starches, and sugars. Because fat is so satisfying, you naturally wind up eating less—without feelings of hunger or deprivation. And natural dietary fat is ultimately the key to feeling better, looking better, thinking clearer, and living longer. Discover all this and more healthy tips in one of the most groundbreaking nutritional books in recent time.