The Functional Nucleus

By way of its clear and logical structure, as well as abundant highresolution illustrations, this is a systematic survey of the players and pathways that control genome function in the mammalian cell nucleus. As such, this handbook and reference ties together recently gained knowledge from a variety of scientific disciplines and approaches, dissecting all major genomic events: transcription, replication, repair, recombination and chromosome segregation. A special emphasis is put on transcriptional control, including genome-wide interactions and non-coding RNAs, chromatin structure, epigenetics and nuclear organization. With its focus on fundamental mechanisms and the associated biomolecules, this will remain essential reading for years to come.

Written and edited by experts in the field, this volume includes contributions discussing the relationship between nuclear structure and function, the various nuclear bodies that have been identified, and the organization of the nuclear lamina and nuclear pore complex.

This book is about the role of calcium and calmodulin in the cell nucleus. Calcium, which is an important second messenger of signal transduction pathways, can also operate in the cell nucleus. Different calcium binding proteins, which are the targets of cellular calcium, have been identified in the nucleus of many different cell types. Prominent among these calcium binding proteins is calmodulin, which appears to be involved in the regulation of major nuclear functions such as gene expression and DNA replication.

This book gives an in-depth overview on nuclear structure and function. It clearly shows that the epigenome and the three-dimensional organization of the nucleus are not independent properties. The intimate relationship between the location and the epigenetic modifications of gene loci is highlighted. Finally, it shows that the complex three-dimensional organization of the nucleus is not just of academic interest: The structure, composition and function of virtually all of the sub-nuclear compartments identified so far can be implicated to a list of human genetic diseases. Hence, a detailed elucidation of how these domains are assembled and function will provide new opportunities for therapeutic intervention in clinical practice.

Within the past two decades, extraordinary new functions for the nucleolus have begun to appear, giving the field a new vitality and generating renewed excitement and interest. These new discoveries include both newly-discovered functions and aspects of its conventional role. The Nucleolus is divided into three parts: nucleolar structure and organization, the role of the nucleolus in ribosome biogenesis, and novel functions of the nucleolus.

Introduces cells, discussing their structure, life cycle, and what they can do.

The nucleus guides the life processes of the cell by directing cellular reproduction, differentiation during development, and metabolism. The study of the structure and function of the nucleus along with its genetic material serves as the foundation for the science of genetics. Principles of Nuclear Structure and Function provides a comprehensive overview of the cell nucleus by illustrating the connection between function and the architecture of the nucleus. Richly illustrated throughout, each chapter includes an overview, detailed examples, summary points, references, and callout boxes highlighting methods and cutting-edge technology. The appendix provides a useful list of related Web sites. Some of the subjects reviewed within Principles of Nuclear Structure and Function include: * Nuclear structure, replication, damage, and repair * Regulation of gene expression * The cell cycle * Meiosis and recombination This timely volume presents functional studies within their proper structural context and is an informative profile of the cell and molecular biology in nuclei and chromatin. For those studying cell biology, along with molecular and cell biologists, geneticists, and reproductive biologists, Principles of Nuclear Structure and Function is a definitive resource. Visit www.wiley.com/cook for supplementary information, including additional Web resources, downloadable figures, and discussion questions.

Acidic Proteins of the Nucleus focuses on the functional role of acidic nuclear proteins in differential gene expression. Historically, these proteins are referred to as acidic in nature because they are insoluble in dilute mineral acids and their amino acid composition shows a preponderance of acidic over basic amino acid residues. After an introduction to DNA-binding proteins and transcriptional control in prokaryotic and eukaryotic systems, the subsequent chapters describe various approaches for isolating, separating, and characterizing acidic nuclear proteins. The core chapters specifically cover the isolation, fractionation, and characterization of acidic nuclear phosphoproteins, and the role of these proteins in cell proliferation, cell differentiation, and cell cycle. The last two chapters address the role of acidic nuclear protein in binding steroid hormones and in gene regulation. Each chapter contains some previously unpublished work and provides recommendations for future research. This book will be a good reference background for researchers of acidic nuclear proteins.

Functional Organization of The Nucleus