Plant Proteomics

There have been several advancements made in high-throughput protein technologies creating immense possibilities for studying proteomics on a large scale. Researchers are exploring various proteomic techniques to unravel the mystery of plant stress tolerance mechanisms. Plant Proteomics: Implications in Growth, Quality Improvement, and Stress Resilience introduces readers to techniques and methodologies of proteomics and explains different physiological phenomena in plants and their responses to various environmental cues and defense mechanisms against pathogens. The main emphasis is on research involving applications of proteomics to understand different aspects of the life cycle of plant species including dormancy, flowering, photosynthetic efficiency, nitrogen assimilation, accumulation of nutritional parameters, secondary metabolite production, reproduction and grain yield as well as signalling responses during abiotic and biotic stresses. The book takes a unique approach, encompassing high throughput and sophisticated proteomic techniques while integrating proteomics with other “omics.” Features: Integrates the branch of proteomics with other “omics” approaches including genomics and metabolomics, giving a holistic view of the overall “omics” approaches. Covers various proteomics approaches for the identification of biological processes, future perspectives, and upcoming applications to identify diverse genes in plants. Presents readers with various proteomics tools for the improvement of plant growth, quality, and resilience against climate change, and pathogen infection. Enables researchers in identifying novel proteins that could be used as target to generate plants with improved traits. Prof. Aryadeep Roychoudhury is currently working as Professor in the Discipline of Life Sciences, Indira Gandhi National Open University, New Delhi, India. Earlier, he served as Assistant Professor at the Post Graduate Department of Biotechnology, St. Xavier’s College (Autonomous), Kolkata, West Bengal, India. He received his B.Sc. (Hons.) in Botany from Presidency College, Kolkata, and M.Sc. in Biophysics and Molecular Biology, University of Calcutta, West Bengal, India. He earned his Ph.D. from Jadavpur University, Kolkata in the area of stress biology in plants. Following his Ph.D. work, he joined as Research Associate (Post doctorate) at the University of Calcutta, pursuing translational research on transgenic rice. He is presently involved in active research in the field of abiotic stress responses in plants with perspectives to the physiology, molecular biology and cell signaling under diverse stress conditions. He has 23 years of research experience in the concerned discipline. Prof. Roychoudhury has handled several government-funded projects as principal investigator and supervised five Ph.D. students as principal investigator. He has published over 250 articles in peer-reviewed journals and chapters in books of international and national repute. He has edited many books with Wiley, Elsevier, and Springer, and has also handled Special Issues as Guest Editor for several renowned international journals. He is a regular reviewer of articles in high-impact, international journals, Life Member of different scientific associations and societies, and the recipient of the Young Scientist Award 2019, conferred upon him by International Foundation for Environment and Ecology, at University of Allahabad, Prayagraj, Uttar Pradesh. His name is included in the Stanford University’s List of World’s Top 2% Influential Scientists.

Plant Proteomics highlights rapid progress in this field, with emphasis on recent work in model plant species, sub-cellular organelles, and specific aspects of the plant life cycle such as signaling, reproduction and stress physiology. Several chapters present a detailed look at diverse integrated approaches, including advanced proteomic techniques combined with functional genomics, bioinformatics, metabolomics and molecular cell biology, making this book a valuable resource for a broad spectrum of readers.

The Special Issue “Plant Proteomics 3.0” was conceived in an attempt to address the recent advancements in as well as limitations of current proteomic techniques and their diverse applications to attain new insights into plant molecular responses to various biotic and abiotic stressors and the molecular bases of other processes. Proteomics’ focus is also related to translational purposes, including food traceability and allergen detection. In addition, bioinformatic techniques are needed for more confident identification, quantitation, data analysis and networking, especially with non-model or orphan plants, including medicinal and meditational plants as well as forest tree species. This Special Issue contains 23 articles, including four reviews and 19 original papers.

This detailed protocol book provides phosphoproteomics techniques currently developed for use in plants, as well as offering a variety of methodologies to analyze different types of plant phosphoproteomic data. Suitable protocols for every stage of the experiment, from protein isolation and phosphopeptides enrichment to large-scale data analysis, have been considered and explored. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Phosphoproteomics: Methods and Protocols serves as an ideal guide for researchers examining phosphoproteomics in the field of plant sciences.

Confidently face the challenges of proteomics research specific to plant science with the information in Plant Proteomics, which will introduce you to the techniques and methodologies required for the study of representative plant species. Read about proteomics studies in Arabidopsis, rice, and legumes and find information about common technologies like mass spectrometry and gel electrophoresis. Discover expression proteomics, functional proteomics, structural proteomics, bioinformatics, and systems biology, understand how to conduct proteomics studies in developing countries and underfunded laboratories, and gain access to guidelines for sample preparation.

This book is a printed edition of the Special Issue "Plant Proteomic Research" that was published in IJMS

As an important tool of systems biology, proteomics has enabled a deep understanding of different plant processes and functions. Complemented with genomic data, computational tools, and improved sample preparation strategies, proteomics has an unprecedented opportunity to characterize plant proteoforms in high spatial and temporal resolution. This special issue of Plant Proteomic Research 4.0 captures the recent advancements in proteomics and addresses the current challenges of plant stress response and resilience in the ever-changing climate. It contains 12 articles, including three reviews and nine original research articles. The three reviews deal with pollen phosphoproteomics, starch biosynthesis-related proteins and posttranslational modifications (PTMs) in rice developing seeds, and PTMs of waxy proteins in rice grain. The nine research articles include three related to temperature, two on water stress, two on salt stress, one on fungal pathogen, and the last one on field-grown potato apoplast proteome. The articles reflect the current frontiers of plant proteomics, focusing on themes of environmental stresses, proteoforms/PTMs, crop species, and new development in data-independent acquisition mass spectrometry. They provide readers insights into current technologies, their utility in understanding plant growth and resilience, as well as directions of proteomics in the frontiers of systems biology and synthetic biology.

Plant diseases, extreme weather caused by climate change, drought and an increase in metals in soil are amongst the major limiting factors of crop production worldwide. They devastate not only food supply but also the economy of a nation. Keeping in view of the global food scarcity, there is, an urgent need to develop crop plants with increased stress tolerance so as to meet the global food demands and to preserve the quality of our planet. In order to do this, it is necessary to understand how plants react and adapt to stress from the genomic and proteomic perspective. Plants adapt to stress conditions by activation of cascades of molecular mechanisms, which result in alterations in gene expression and synthesis of protective proteins/compounds. From the perception of the stimulus to transduction of the signal, followed by an appropriate response, the plants employ a complex network of primary and secondary messenger molecules. Cell signaling is the component of a complex system of communication that directs basic cellular activities and synchronizes cell actions. Cells exercise a large number of noticeably distinct signaling pathways to regulate their activity. In order to contend with different environmental adversities plants have developed a series of mechanisms at the physiological, cellular and molecular level. This two volume set takes an in-depth look at the Stress Signaling in Plants from a uniquely genomic and proteomics perspective. Stress Signaling in Plants offers a comprehensive treatise on the Chapter, covering all of the signaling pathways and mechanisms that have been researched so far. Each chapter provides in-depth explanation of what we currently know of a particular aspect of stress signaling and where we are headed. All authors have currently agreed and abstracts have been complied for the first volume, due out midway through 2012. We aim to have the second volume out at the beginning of 2013.​