Plant Pattern Recognition Receptors

"This volume covers protocols on techniques ranging from MAMP isolations from diverse microorganisms, PRR identifications from different plant species, MAMP-PRR binding, and a series of signaling responses and events revealed by various biochemical, cellular, genetic and bioinformatic tools. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Pattern Recognition Receptors: Methods and Protocolsaims to ensure successful results in the further study of this vital field." -- OCLC.

This volume covers protocols on techniques ranging from MAMP isolations from diverse microorganisms, PRR identifications from different plant species, MAMP-PRR binding, and a series of signaling responses and events revealed by various biochemical, cellular, genetic and bioinformatic tools. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Pattern Recognition Receptors: Methods and Protocols aims to ensure successful results in the further study of this vital field.

This book presents the ways and means to switch on plant immune signaling systems using PAMP-PIMP-PRR signaling complex for crop disease management. It also describes bioengineering approaches to develop transgenic plants expressing enhanced disease resistance using genes encoding PAMPs, PRRs and transcription factors and genes involved in generation of PIMPs/HAMPs. It also discusses recent commercial development of PAMP products to switch on plant innate immunity for crop disease management. These unique approaches have been described with more than 100 figures and illustrations and these would make this book attractive for researchers and students to buy this book.

The volume III of the book presents the ways and means to manipulate the signals and signaling system to enhance the expression of plant innate immunity for crop disease management. It also describes bioengineering approaches to develop transgenic plants expressing enhanced disease resistance using plant immunity signaling genes. It also discusses recent commercial development of biotechnological products to manipulate plant innate immunity for crop disease management. Engineering durable nonspecific resistance to phytopathogens is one of the ultimate goals of plant breeding. However, most of the attempts to reach this goal fail as a result of rapid changes in pathogen populations and the sheer diversity of pathogen infection mechanisms. Recently several bioengineering and molecular manipulation technologies have been developed to activate the ‘sleeping’ plant innate immune system, which has potential to detect and suppress the development of a wide range of plant pathogens in economically important crop plants. Enhancing disease resistance through altered regulation of plant immunity signaling systems would be durable and publicly acceptable. Strategies for activation and improvement of plant immunity aim at enhancing host’s capability of recognizing invading pathogens, boosting the executive arsenal of plant immunity, and interfering with virulence strategies employed by microbial pathogens. Major advances in our understanding of the molecular basis of plant immunity and of microbial infection strategies have opened new ways for engineering durable resistance in crop plants.

Sequencing projects have revealed the presence of at least several hundred receptor kinases in a typical plant genome. Receptor kinases are therefore the largest family of primary signal transducers in plants, and their abundance suggests an immense signaling network that we have only just begun to uncover. Recent research findings indicate that individual receptor kinases fulfill important roles in growth and development, in the recognition of pathogens and symbionts or, in a few examples, in both growth and defense. This volume will focus on the roles of receptor kinases, their signaling pathways, and the ways in which these important signaling proteins are regulated.

Pattern-recognition receptors (PRRs) localized at the plasma membrane of plant cells monitor the apoplastic space for pathogen-associated molecular patterns (PAMPs), damage-associated molecular patterns (DAMPs) and apoplastic effectors. In my thesis research I studied different aspects of the two Arabidopsis PRRs EFR and FLS2. Both EFR and FLS2 are leucine-rich repeat (LRR) receptor-like kinases (RLKs). First I cloned, sequenced and functionally tested in chimeric receptors the LRR-encoding domains of nine novel EFR alleles from seven Brassicaceae species. The sequences were subjected to Repeat Conservation Mapping (RCM), a computational program developed in our lab that identifies conserved patches on the predicted surface of LRR domains. This analysis revealed conserved clusters in the LRR domain of EFR, which are candidate functionally important sites. Additional work used RCM to compare the last seven LRRs of the BAK1-interacting LRR-RLKs EFR, FLS2, BRI1 and PEPR1, and revealed a conserved putative BAK1 ectodomain interaction site. However, site-directed mutagenesis of the putative BAK1 interaction site in the FLS2 ectodomain did not disrupt interaction with BAK1 and analogous mutagenesis in EFR resulted in receptor maturation defects. Hence this conserved LRR C-terminal region apparently has functions other than mediating interactions with BAK1. I then performed In vivo tests of the newly published FLS2-BAK1-flg22 ectodomain co-crystal structure, with the resulting data supporting models in which FLS2 signaling is initiated by formation of a ligand mediated FLS2 - BAK1 ectodomain complex. In an effort to mold our constantly growing knowledge about the plant immune system into agriculturally useful tools to improve crop health, I also explored two approaches to engineer novel PRRs. The first approach sought to use yeast surface display to evolve the endogenous LRR domains of EFR and FLS2 towards novel ligand binding capacities. The second approach used a LRR-based variable lymphocyte receptor (VLR) of Petromyzon marinus as template for rational design of novel ligand binding sites in FLS2. Both approaches are compromised by the subsequently discovered finding that PRR activation requires a SERK protein ectodomain as a co-receptor. The research projects presented here further elucidate PRR signaling activation and present possible technologies for engineering novel PRRs to improve crop health.

The second edition of Avian Immunology provides an up-to-date overview of the current knowledge of avian immunology. From the ontogeny of the avian immune system to practical application in vaccinology, the book encompasses all aspects of innate and adaptive immunity in chickens. In addition, chapters are devoted to the immunology of other commercially important species such as turkeys and ducks, and to ecoimmunology summarizing the knowledge of immune responses in free-living birds often in relation to reproductive success. The book contains a detailed description of the avian innate immune system, encompassing the mucosal, enteric, respiratory and reproductive systems. The diseases and disorders it covers include immunodepressive diseases and immune evasion, autoimmune diseases, and tumors of the immune system. Practical aspects of vaccination are examined as well. Extensive appendices summarize resources for scientists including cell lines, inbred chicken lines, cytokines, chemokines, and monoclonal antibodies. The world-wide importance of poultry protein for the human diet, as well as the threat of avian influenza pandemics like H5N1 and heavy reliance on vaccination to protect commercial flocks makes this book a vital resource. This book provides crucial information not only for poultry health professionals and avian biologists, but also for comparative and veterinary immunologists, graduate students and veterinary students with an interest in avian immunology. With contributions from 33 of the foremost international experts in the field, this book provides the most up-to-date review of avian immunology so far Contains a detailed description of the avian innate immune system reviewing constitutive barriers, chemical and cellular responses; it includes a comprehensive review of avian Toll-like receptors Contains a wide-ranging review of the "ecoimmunology" of free-living avian species, as applied to studies of population dynamics, and reviews methods and resources available for carrying out such research

Plant proteases are involved in most aspects of plant physiology and development, playing key roles in the generation of signaling molecules and as regulators of essential cellular processes such as cell division and metabolism. They take part in important pathways like protein turnover by the degradation of misfolded proteins and the ubiquitin-proteasome pathway, and they are responsible for post-translational modifications of proteins by proteolysis at highly specific sites. Proteases are also implicated in a great variety of environmentally controlled processes, including mobilization of storage proteins during seed germination, development of seedlings, senescence, programmed cell death and defense mechanisms against pests and pathogens. However, in spite of their importance, little is known about the functions and mode of actions of specific plant proteases. This Research Topic collects contributions covering diverse aspects of plant proteases research.

Annual Plant Reviews, Volume 34 Molecular Aspects of Plant Disease Resistance Edited by Jane Parker In recent years, our understanding of the mechanisms involved in plant resistance to disease has seen major advances. This important new volume in Wiley-Blackwell’s Annual Plant Reviews provides cutting edge reviews on major aspects of plant immunity from many of the world's leading researchers in the area. Coverage includes: • Establishment of disease by microbial pathogens • Genomic approaches to understanding host-pathogen interactions • Local and systemic resistance signalling • Activities of small bioactive molecules • Plant-insect ecology This exciting volume is essential reading for all those studying plant-pathogen interactions including plant and agricultural scientists, molecular biologists, geneticists and microbiologists. Libraries in all universities and research establishments where biological and agricultural sciences are studied and taught should have copies of this important volume on their shelves. About the Editor Dr Jane Parker is a Group Leader in the Department of Plant-Microbe Interactions at The Max-Planck Institute of Plant Breeding Research, Cologne and Associate Professor at The Institute of Genetics, University of Cologne, Germany. Also Available Annual Plant Reviews, Volume 33 Intracellular Signaling in Plants Edited by Zhenbiao Yang Print: 9781405160025 Annual Plant Reviews, Volume 32 Cell Cycle Control and Plant Development Edited by Dirk Inzé Print: 9781405150439 Online: 9780470988923 Annual Plant Reviews, Volume 31 Plant Mitochondria Edited by David Logan Print: 9781405149396 Online: 9780470986592 Annual Plant Reviews, Volume 30 Light and Plant Development Edited by Garry C. Whitelam and Karen J. Halliday Print: 9781405145381 Online: 9780470988893