Nonlinear Optical Microscopy

The Handbook of Biomedical Nonlinear Optical Microscopy provides comprehensive treatment of the theories, techniques, and biomedical applications of nonlinear optics and microscopy for cell biologists, life scientists, biomedical engineers, and clinicians. The chapters are separated into basic and advanced sections, and provide both textual and graphical illustrations of all key concepts. The more basic sections are aimed at life scientists without advanced training in physics and mathematics, and tutorials are provided for the more challenging sections. The first part of the Handbook introduces the historical context of nonlinear microscopy. The second part presents the nonlinear optical theory of two- and multiphoton excited fluorescence (TPE, MPE) spectroscopy, second and third harmonic generation (SHG, THG) spectroscopy, and coherent anti-Stokes Raman spectroscopy (CARS). The third part introduces modern microscopic and spectroscopic instrumentation and techniques that are based on nonlinear optics. The fourth part provides key applications of nonlinear microscopy to the biomedical area: neurobiology, immunology, tumor biology, developmental biology, dermatology, and cellular metabolism. There are also chapters on nonlinear molecular probes, cellular damage, and nanoprocessing.

Foundations of Nonlinear Optical Microscopy Concise yet comprehensive resource presenting the foundations of nonlinear optical microscopy Foundations of Nonlinear Optical Microscopy brings together all relevant principles of nonlinear optical (NLO) microscopy, presenting NLO microscopy within a consistent framework to allow for the origin of the signals and the interrelation between different NLO techniques to be understood. The text provides rigorous yet practical derivations, which amount to expressions that can be directly related to measured values of resolution, sensitivity, and imaging contrast. The book also addresses typical questions students ask, and answers them with clear explanations and examples. Readers of this book will develop a solid physical understanding of NLO microscopy, appreciate the advantages and limitations of each technique, and recognize the exciting possibilities that lie ahead. Foundations of Nonlinear Optical Microscopy covers sample topics such as: Light propagation, focusing of light, pulses of light, classical description of light-matter interactions, and quantum mechanical description of light-matter interactions Molecular transitions, selection rules, signal radiation, and detection of light Multi-photon fluorescence and pump-probe microscopy Harmonic generation, sum-frequency generation, and coherent Raman scattering Senior undergraduate and graduate students in chemistry, physics, and biomedical engineering, along with students of electrical engineering and instructors in both of these fields, can use the information within Foundations of Nonlinear Optical Microscopy and the included learning resources to gain a concise yet comprehensive overview of the subject.

Ideal for cell biologists, life scientists, biomedical engineers, and clinicians, this handbook provides comprehensive treatment of the theories, techniques, and biomedical applications of nonlinear optics and microscopy.

With contributions by numerous experts

Second-harmonic generation (SHG) microscopy has shown great promise for imaging live cells and tissues, with applications in basic science, medical research, and tissue engineering. Second Harmonic Generation Imaging offers a complete guide to this optical modality, from basic principles, instrumentation, methods, and image analysis to biomedical a

Presents a clear systematic molecular-based description of nonlinear optical polarization analysis of chemical and biological assemblies.

The book is designed to serve as a textbook for courses offered to upper-undergraduate students enrolled in physics. The first edition of this book was published in 2014. As there is a demand for the next edition, it is quite natural to take note of the several advances that have occurred in the subject over the past five years and to decide which of these are appropriate for inclusion at the textbook level, given the fundamental nature and the significance of the subject area. This is the prime motivation for bringing out a revised second edition. Among the newer mechanisms and materials, the book introduces the super-continuum generation, which arises from an excellent interplay of the various mechanisms of optical nonlinearity. The topics covered in this book are quantum mechanics of nonlinear interaction of matter and radiation, formalism and phenomenology of nonlinear wave mixing processes, optical phase conjugation and applications, self-focusing and self-phase modulation and their role in pulse modification, nonlinear absorption mechanisms, and optical limiting applications, photonic switching and bi-stability, and physical mechanisms leading to a nonlinear response in a variety of materials. This book has emerged from an attempt to address the requirement of presenting the subject at the college level. This textbook includes rigorous features such as the elucidation of relevant basic principles of physics; a clear exposition of the ideas involved at an appropriate level; coverage of the physical mechanisms of non-linearity; updates on physical mechanisms and emerging photonic materials and emphasis on the experimental study of nonlinear interactions. The detailed coverage and pedagogical tools make this an ideal textbook for students and researchers enrolled in physics and related courses.

Ultrafast lasers allow high-precision imaging and manipulation for biological and medical applications. Nonlinear optical microscopy has provided researchers with unique possibilities of three-dimensional imaging of biological cells and tissues. Nonlinear optical imaging technique is a rapidly emerging research area with widespread fundamental research and clinical applications. Nonlinear optical imaging allows both structural and functional imaging with cellular level resolution imaging in biological systems. The introduction of endogenous or exogenous probes can selectively enhance contrast for molecular targets in a living cell as well as supply functional information on processes. With the aim to control nonlinear optical processes and to obtain functional images, nonlinear optical processes can be controlled by photo-controlled probes and/or parameters of ultrafast laser pulses, such as time, space, polarization, and phase. This book gives an overview of the nonlinear optical process by ultrafast laser pulses and explains how the basics of nonlinear optical microscopy led to the most advanced techniques of photo-controlled nonlinear optical microscopy.