International Symposium On Analytical Applications Of Bioluminescence And Chemiluminescence

Bioluminescence and Chemiluminescence: Basic Chemistry and Analytical Applications is a compendium of papers presented at the second International Symposium on Analytical Applications of Bioluminescene and Chemiluminescence in San Diego, California on August 26-28, 1980. Part I deals with chemilunescence and excited states including topics on the spectroscopy of the solvent cage — generation and characteristics of the excited state and the three features of chemi- and bioluminescence. Part II deals with events prior to producing luminol-dependent chemiluminescence; this part also examines the effects of stimulants on membrane potential. Part III discusses bacterial bioluminescence and analyzes the properties of a lumazine protein from a bioluminescent bacterium. This part also analyzes accessory enzymes responsible for such bacterial bioluminescence. Part IV examines the chemistry of firefly bioluminescence and presents the formula of three reactions catalyzed by firefly luciferase. Part V analyzes bioluminescence from other sources such as the earthworm and land snails. Part VI discusses the applications of bioluminescence in clinical chemistry, soil science, and marine biology. Part VII deals with the future uses of bioluminescence, while Part VIII is an abstract of other papers dealing with this subject. This collection can be helpful for biologists, zoologists, micro-biologists, marine biologists, and researchers dealing with bio-chemistry and related disciplines.

In the last decade, great advances have been made in fundamental research and in the applications of bioluminescence and chemiluminescence. These techniques have become vital tools for laboratory analysis. Bioluminescence imaging has emerged as a powerful new optical imaging technique, offering real-time monitoring of spatial and temporal progression of biological processes in living animals. Bioluminescence resonance energy transfer (BRET) methodology has also emerged as a powerful technique for the study of protein-protein interactions. Luciferase reporter gene technology facilitates monitoring of gene expression and is used to probe molecular mechanisms in the regulation of gene expression. Chemiluminescence detection and analysis have also found diverse applications in life science research; for example, chemiluminescent labels and substrates are now widely used in immunoassay and nucleic acid probe-based assays. The latest advances in this exciting field, from fundamental research to cutting-edge applications, are explored in this most recent volume of the biannual symposium series, the Proceedings of the 15th International Symposium on Bioluminescence and Chemiluminescence. The volume highlights advances in fundamental knowledge of luciferase-based bioluminescence, photoprotein-based bioluminescence, fundamental aspects and applications of chemiluminescence, luminescence imaging, fluorescence quantum dots and other inorganic fluorescent materials, phosphorescence and ultraweak luminescence, and instrumentation for measurement and imaging of luminescence.

This comprehensive overview of chemiluminescence and bioluminescence covers historical developments, fundamental principles, recent advances, instrumentation, and applications.