Experimental Techniques In Host Plant Resistance

The earliest land-plants evolved around 450 million years ago from aquatic plants devoid of vascular systems. The diversification of flowering plants (angiosperms) during the Cretaceous period is associated with speciation in insects. Early insect herbivores were mandibulate, but the evolution of vascular plants led to the co-evolution of other forms of herbivory, such as leaf feeding, sap-sucking, leaf mining, tissue borer, gall forming and nectar-feeding. Plant defense against biotic stress is an adaptive evolution by plants to increase their fitness. Plants use a variety of strategies to defend against damage caused by herbivores. Plant defense mechanisms are either inbuilt or induced. Inbuilt mechanisms are always present within the plant, while induced defenses are produced or mobilized to the site where a plant is injured. Induced defense mechanisms include morphological, physiological changes and production of secondary metabolites. Host plant resistance (HPR) is one of the eco-friendly methods of pest management. It protects the crop by making it less suitable or tolerant to the pest. While books on theoretical aspects of HPR are available, an exclusive book on the practical aspects is lacking. There is a wide gap between the theory and the experimental procedures required for conducting studies on plant resistance for the post graduate students and young researchers. A dire need for a book on practical aspects was strongly felt. Initially a practical manual was prepared which eventually evolved into the present book. We hope this book provides information on major aspects of screening crop germplasm, sampling techniques, genetic and biochemical basis of HPR, behavioural studies on pheromone and plant volatiles, and some of the recent approaches in HPR. Further, the references provide the scientific articles and books as additional information to readers and workers alike.

The earliest land-plants evolved around 450 million years ago from aquatic plants devoid of vascular systems. The diversification of flowering plants (angiosperms) during the Cretaceous period is associated with speciation in insects. Early insect herbivores were mandibulate, but the evolution of vascular plants led to the co-evolution of other forms of herbivory, such as leaf feeding, sap-sucking, leaf mining, tissue borer, gall forming and nectar-feeding. Plant defense against biotic stress is an adaptive evolution by plants to increase their fitness. Plants use a variety of strategies to defend against damage caused by herbivores. Plant defense mechanisms are either inbuilt or induced. Inbuilt mechanisms are always present within the plant, while induced defenses are produced or mobilized to the site where a plant is injured. Induced defense mechanisms include morphological, physiological changes and production of secondary metabolites. Host plant resistance (HPR) is one of the eco-friendly methods of pest management. It protects the crop by making it less suitable or tolerant to the pest. While books on theoretical aspects of HPR are available, an exclusive book on the practical aspects is lacking. There is a wide gap between the theory and the experimental procedures required for conducting studies on plant resistance for the post graduate students and young researchers. A dire need for a book on practical aspects was strongly felt. Initially a practical manual was prepared which eventually evolved into the present book. We hope this book provides information on major aspects of screening crop germplasm, sampling techniques, genetic and biochemical basis of HPR, behavioural studies on pheromone and plant volatiles, and some of the recent approaches in HPR. Further, the references provide the scientific articles and books as additional information to readers and workers alike.

Most books on epidemiology have treated the subject from a statistical, mathematical or computer applicational point of view. However, experiments must be performed first to provide the data for models which in turn can then be proven by further experimentation. This mutual interplay of theory and empirics gives epidemiology its scientific thrust and charm. This book provides a choice of methods for varying applications and objectives, covering all important aspects for the designing of experiments. Furthermore, the reader is supplied with solutions to his experimental problems and many "tricks of the trade". The newcomer to the field will also profit by this methodology guide.

Molecular Host Plant Resistance to Pests examines environmentally safe and integrated techniques for effective pest management. Offering more than 1500 references for further exploration of the topic, this reference details the bioactivity, biosynthetic pathways, mechanisms of action, and genetic regulation for improved methods of crop protection and analyzes host plant resistance mechanisms for development of enhanced insect management programs and agricultural ecosystems. This reference discusses the morphological and phenological bases of plant resistance and the current molecular studies on the chemistry, classification, and occurrence of a variety of chemical constituents.

Molecular Host Plant Resistance to Pests examines environmentally safe and integrated techniques for effective pest management. Offering more than 1500 references for further exploration of the topic, this reference details the bioactivity, biosynthetic pathways, mechanisms of action, and genetic regulation for improved methods of crop protection and analyzes host plant resistance mechanisms for development of enhanced insect management programs and agricultural ecosystems. This reference discusses the morphological and phenological bases of plant resistance and the current molecular studies on the chemistry, classification, and occurrence of a variety of chemical constituents.

This book reviews and synthesizes the recent advances in exploiting host plant resistance to insects, highlighting the role of molecular techniques in breeding insect resistant crops. It also provides an overview of the fascinating field of insect-plant relationships, which is fundamental to the study of host-plant resistance to insects. Further, it discusses the conventional and molecular techniques utilized/useful in breeding for resistance to insect-pests including back-cross breeding, modified population improvement methods for insect resistance, marker-assisted backcrossing to expedite the breeding process, identification and validation of new insect-resistance genes and their potential for utilization, genomics, metabolomics, transgenesis and RNAi. Lastly, it analyzes the successes, limitations and prospects for the development of insect-resistant cultivars of rice, maize, sorghum and millet, cotton, rapeseed, legumes and fruit crops, and highlights strategies for management of insect biotypes that limit the success and durability of insect-resistant cultivators in the field. Arthropod pests act as major constraints in the agro-ecosystem. It has been estimated that arthropod pests may be destroying around one-fifth of the global agricultural production/potential production every year. Further, the losses are considerably higher in the developing tropics of Asia and Africa, which are already battling severe food shortage. Integrated pest management (IPM) has emerged as the dominant paradigm for minimizing damage by the insects and non-insect pests over the last 50 years. Pest resistant cultivars represent one of the most environmentally benign, economically viable and ecologically sustainable options for utilization in IPM programs. Hundreds of insect-resistant cultivars of rice, wheat, maize, sorghum, cotton, sugarcane and other crops have been developed worldwide and are extensively grown for increasing and/or stabilizing crop productivity. The annual economic value of arthropod resistance genes developed in global agriculture has been estimated to be greater than US$ 2 billion Despite the impressive achievements and even greater potential in minimizing pest- related losses, only a handful of books have been published on the topic of host-plant resistance to insects. This book fills this wide gap in the literature on breeding insect- resistant crops. It is aimed at plant breeders, entomologists, plant biotechnologists and IPM experts, as well as those working on sustainable agriculture and food security.

Insect science is fast changing as insects are evolving to a plethora of newer chemical molecules, climate change, management tactics and transformation of the landscapes. Through the International Conference, the editors have attempted to gather together newer aspects of Insect Sciences like Insect Taxonomy, DNA Barcoding, Physiology, Toxicology, Vectors and their Management, Molecular Biology, RNA interference in Pest Management, Semiochemicals and Pest Management using Host Plant Resistance and Biological Control appropriated especially for the developing world. Both basic and applied aspects of insect science have been included to stimulate comprehensive studies on insect science. The book not only deals with insect science but also environmental and ecological aspects in the hope that the book will be of immense use to students, researchers, extension workers, planners, administrators, farmers and other end users. The Chapters on diversified aspects of Insect Science are contributed by leading scientists for the coming 21st century in which entomology is witnessing a dramatic advancement in management of pests through in-depth investigations. The dimensions of Insect Science covered in the book are pest management approaches that can be adopted worldwide with ascent on sustainability.