Electron Paramagnetic Resonance In Biochemistry And Medicine

Electron Paramagnetic Resonance (EPR) spectroscopy - also sometimes termed Electron Spin Resonance spectroscopy - has manifold potential uses in biochemistry and medicine. The paramount importance of EPR spectroscopy applied to biological tissues and fluids is that it identifies the changes in redox processes that contribute to disease. EPR spectroscopy has come a long way from its original use to detect malignant tumors. For example, the development and later refinement of methods of low-temperature registration of biological tissues widened the scope of EPR spectroscopy. Innovations made possible by the introduction of spin labels, probes, and traps made EPR spectroscopy ever more applicable to biochemistry and medicine, to the point where in vivo studies are being carefully considered. This comprehensive book discusses spectra of many tissues and bodily fluids, and the quantitative nature of paramagnetic centers in both normal individuals and patients suffering from a variety of diseases. Special attention is given to the EPR examination of bio-molecules, such as enzymes, polypeptides, vitamins, lipids, hydrocarbons, etc., which play an essential role in human activity. This book will be of great interest to physicians specializing in many different areas. Similarly, biologists, biochemists, biophysicists, and chemists will find this book very useful. It has also been written so that it may be used as a textbook at graduate level.

Biomedical EPR – Part A focuses on applications of EPR spectroscopy in the areas of free radicals, metals, medicine, and physiology. The book celebrates the 70th birthday of Prof. James S. Hyde, Medical College of Wisconsin, and his contributions to this field. Chapters are written to provide introductory material for new-comers to the field which lead into up-to-date reviews that provide perspective on the wide range of questions that can be addressed by EPR. Key Features: Free Radicals in Medicine Radicals in vivo and in Model Systems, and their Study by Spin Trapping In vivo EPR, including Oximetry and Imaging Time Domain EPR at Radio Frequencies EPR of Copper Complexes: Motion and Frequency Dependence Time Domain EPR and Electron Spin Echo Envelope Modulation

Biomedical EPR – Part A focuses on applications of EPR spectroscopy in the areas of free radicals, metals, medicine, and physiology. The book celebrates the 70th birthday of Prof. James S. Hyde, Medical College of Wisconsin, and his contributions to this field. Chapters are written to provide introductory material for new-comers to the field which lead into up-to-date reviews that provide perspective on the wide range of questions that can be addressed by EPR. Key Features:Free Radicals in Medicine Radicals in vivo and in Model Systems, and their Study by Spin Trapping In vivo EPR, including Oximetry and Imaging Time Domain EPR at Radio Frequencies EPR of Copper Complexes: Motion and Frequency Dependence Time Domain EPR and Electron Spin Echo Envelope Modulation About the Editors: Prof. Sandra S. Eaton is John Evans Professor in the Department of Chemistry and Biochemistry at the University of Denver. Her research interests include distance measurements in proteins, EPR of metal ions in biological systems, electron spin relaxation times, and EPR instrumentation. The Eatons co-organize an annual EPR Symposium in Denver. Prof. Gareth R. Eaton is John Evans Professor in the Department of Chemistry and Biochemistry at the University of Denver. His research interests include EPR instrumentation, distance measurements in proteins, EPR of metal ions in biological systems, and electron spin relaxation times. Dr. Lawrence J. Berliner is currently Professor and Chair of the Department of Chemistry and Biochemistry at the University of Denver after retiring from Ohio State University, where he spent a 32-year career in the area of biological magnetic resonance (EPR and NMR). He is the Series Editor for Biological Magnetic Resonance, which he launched in 1979.

Biomedical EPR – Part B focuses on applications of EPR techniques and instrumentation, with applications to dynamics. The book celebrates the 70th birthday of Prof. James S. Hyde, Medical College of Wisconsin, and his contributions to this field. Chapters are written to provide introductory material for new-comers to the field that lead into up-to-date reviews that provide perspective on the wide range of questions that can be addressed by EPR. Key Features: EPR Techniques including Saturation Recovery, ENDOR, ELDOR, and Saturation Transfer Instrumentation Innovations including Loop Gap Resonators, Rapid Mixing, and Time Locked Sub-Sampling Motion in Biological Membranes Applications to Structure Determination in Proteins Discussion of Trends in EPR Technology and Prognosis for the Future

Annotation Electron Paramagnetic Resonance (EPR) highlights major developments in this area, with results being set into the context of earlier work and presented as a set of critical yet coherent overviews. The topics covered describe contrasting types of application, ranging from biological areas such as EPR studies of free-radical reactions in biology and medically-related systems, to experimental developments and applications involving EPR imaging, the use of very high fields, and time-resolved methods. Critical and up-to-the-minute reviews of advances involving the design of spin-traps, advances in spin-labelling, paramagnetic centres on solid surfaces, exchange-coupled oligomers, metalloproteins and radicals in flavoenzymes are also included. As EPR continues to find new applications in virtually all areas of modern science, including physics, chemistry, biology and materials science, this series caters not only for experts in the field, but also those wishing to gain a general overview of EPR applications in a given area.

Electron Paramagnetic Resonance Investigations of Biological Systems by Using Spin Labels, Spin Probes, and Intrinsic Metal Ions Part A & B, are the latest volumes in the Methods in Enzymology series, continuing the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods centered on the use of Electron Paramagnetic Resonance (EPR) techniques to study biological structure and function. Timely contribution that describes a rapidly changing field Leading researchers in the field Broad coverage: Instrumentation, basic theory, data analysis, and applications

Biomedical EPR – Part A focuses on applications of EPR spectroscopy in the areas of free radicals, metals, medicine, and physiology. The book celebrates the 70th birthday of Prof. James S. Hyde, Medical College of Wisconsin, and his contributions to this field. Chapters are written to provide introductory material for new-comers to the field which lead into up-to-date reviews that provide perspective on the wide range of questions that can be addressed by EPR. Key Features: Free Radicals in Medicine Radicals in vivo and in Model Systems, and their Study by Spin Trapping In vivo EPR, including Oximetry and Imaging Time Domain EPR at Radio Frequencies EPR of Copper Complexes: Motion and Frequency Dependence Time Domain EPR and Electron Spin Echo Envelope Modulation

Electron Paramagnetic Resonance Investigations of Biological Systems by Using Spin Labels, Spin Probes, and Intrinsic Metal Ions Part A & B, are the latest volumes in the Methods in Enzymology series, continuing the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods centered on the use of Electron Paramagnetic Resonance (EPR) techniques to study biological structure and function. Timely contribution that describes a rapidly changing field Leading researchers in the field Broad coverage: Instrumentation, basic theory, data analysis, and applications

This book offers a pragmatic guide to navigating through the complex maze of EPR/ESR spectroscopy fundamentals, techniques, and applications. Written for the scientist who is new to EPR spectroscopy, the editors have prepared a volume that de-mystifies the basic fundamentals without weighting readers down with detailed physics and mathematics, and then presents clear approaches in specific application areas. The first part presents basic fundamentals and advantages of electron paramagnetic resonance spectrscopy. The second part explores severalapplication areas including chemistry, biology, medicine, materials and geology. A frequently-asked-questions sections focuses on practicalquestions, such as the size of sample, etc. It's an ideal, hands-on reference for chemists and researchers in the pharmaceutical and materials (semiconductor) industries who are looking for a basic introduction to EPR spectroscopy.

Metalloproteins comprise approximately 30% of all known proteins, and are involved in a variety of biologically important processes, including oxygen transport, biosynthesis, electron transfer, biodegradation, drug metabolism, proteolysis, and hydrolysis of amides and esters, environmental sulfur and nitrogen cycles, and disease mechanisms. EPR spectroscopy has an important role in not only the geometric structural characterization of the redox cofactors in metalloproteins but also their electronic structure, as this is crucial for their reactivity. The advent of x-ray crystallographic snapshots of the active site redox cofactors in metalloenzymes in conjunction with high-resolution EPR spectroscopy has provided detailed structural insights into their catalytic mechanisms. This volume was conceived in 2005 at the Rocky Mountain Conference on Analytical Chemistry (EPR Symposium) to highlight the importance of high-resolution EPR spectroscopy to the structural (geometric and electronic) characterization of redox active cofactors in metalloproteins. We have been fortunate to have enlisted internationally recognized experts in this joint venture to provide the scientific community with an overview of high-resolution EPR and its application to metals in biology. This volume, High-Resolution EPR: Applications to Metalloenzymes and Metals in Medicine, covers high-resolution EPR methods, iron proteins, nickel and copper enzymes, and metals in medicine. An eloquent synopsis of each chapter is provided by John Pilbrow in the Introduction. A second volume, Metals in Biology: Applications of High-Resolution EPR to Metalloenzymes, will appear later this year covering the complement of other metalloproteins. One of the pioneers in the development of pulsed EPR and its application to metalloproteins was Arthur Schweiger, whose contribution we include in this volume. Unfortunately, he passed away suddenly during the preparation of this volume. The editors and coauthors are extremely honored to dedicate this volume to the memory of Arthur Schweiger in recognition of his technical advances and insights into pulsed EPR and its application to metalloproteins. Arthur was extremely humble and treated everyone with equal respect. He was a gifted educator with an ability to explain complex phenomena in terms of simple intuitive pictures, had a delightful personality, and continues to be sadly missed by the community. It is an honor for the editors to facilitate the dissemination of these excellent contributions to the scientific community. Suggestions for future volumes are always appreciated.