Detection Of Deoxyribonucleic Acid Damage Induced By Metal Ion Interactions And Repair Of Metal Ion Deoxyribonucleic Acid Cross Links
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Author | : Neal Esau |
Publisher | : |
Total Pages | : 220 |
Release | : 2010 |
Genre | : DNA damage |
ISBN | : |
Download Detection of Deoxyribonucleic Acid Damage Induced by Metal Ion Interactions and Repair of Metal Ion Deoxyribonucleic Acid Cross-links Book in PDF, Epub and Kindle
"There are many forms of DNA damage. One of these, DNA-metal cross-links are particularly problematic as they not only affect the tautomeric structure of DNA, but can also inhibit enzymatic activity and block amplification with DNA polymerases. Since the inability to amplify DNA can affect many fields of genetic research, it is necessary to find ways to identify this damage and repair the DNA. Copper is one metal that can form metal-DNA cross-links and can seriously affect the recovery and analysis of degraded DNA from forensic or archaeological material. In this research DNA-copper cross-links were generated for the development of a repair method and applied to an archaeological sample. The DNA-copper adducts were identified through the use of gas chromatography-mass spectrometry on several templates of different complexity: single deoxyribonucleotides, a synthetic 22 base pair double stranded DNA fragment, modern amplified DNA, and an ancient extract with naturally occurring copper cross-links. A number of chemicals were considered for direct reversal repair of copper-DNA cross-links of which ethylenediamine was successful. Treatment of all the templates with ethylenediamine resulted in the repair of the nucleobase, specifically guanine which is the most susceptible to copper cross-link formation. The success of the direct reversal repair was verified using GC-MS based on expected retention time and the identification of ion fingerprints. The amount of copper-DNA adducts measured in each template varied greatly as did the success of the direct reversal repair although repair was evident in all samples."--Abstract.
Author | : |
Publisher | : |
Total Pages | : 1732 |
Release | : 1995 |
Genre | : Medicine |
ISBN | : |
Download Cumulated Index Medicus Book in PDF, Epub and Kindle
Author | : |
Publisher | : |
Total Pages | : 2432 |
Release | : 2004 |
Genre | : Medicine |
ISBN | : |
Download Index Medicus Book in PDF, Epub and Kindle
Vols. for 1963- include as pt. 2 of the Jan. issue: Medical subject headings.
Author | : E. C. Foulkes |
Publisher | : CRC Press |
Total Pages | : 218 |
Release | : 1990-09-06 |
Genre | : Medical |
ISBN | : |
Download Biological Effects of Heavy Metals Book in PDF, Epub and Kindle
V.1. [without special title] -- v.2. Metal carcinogenesis.
Author | : |
Publisher | : |
Total Pages | : 744 |
Release | : 1975 |
Genre | : Nuclear energy |
ISBN | : |
Download Nuclear Science Abstracts Book in PDF, Epub and Kindle
Author | : National Institutes of Health (U.S.). Division of Research Grants |
Publisher | : |
Total Pages | : 1212 |
Release | : 1975 |
Genre | : Medicine |
ISBN | : |
Download Research Grants Index Book in PDF, Epub and Kindle
Author | : |
Publisher | : |
Total Pages | : 440 |
Release | : 1992 |
Genre | : Radiology |
ISBN | : |
Download British Journal of Radiology Book in PDF, Epub and Kindle
Author | : |
Publisher | : |
Total Pages | : 1224 |
Release | : |
Genre | : Medicine |
ISBN | : |
Download Research Awards Index Book in PDF, Epub and Kindle
Author | : |
Publisher | : |
Total Pages | : 778 |
Release | : 1995 |
Genre | : Nucleic acids |
ISBN | : |
Download Nucleic Acids Abstracts Book in PDF, Epub and Kindle
Author | : Wolfram Siede |
Publisher | : CRC Press |
Total Pages | : 871 |
Release | : 2005-09-19 |
Genre | : Medical |
ISBN | : 0849352681 |
Download DNA Damage Recognition Book in PDF, Epub and Kindle
Stands as the most comprehensive guide to the subject-covering every essential topic related to DNA damage identification and repair. Covering a wide array of topics from bacteria to human cells, this book summarizes recent developments in DNA damage repair and recognition while providing timely reviews on the molecular mechanisms employe