Desoxyribonucleic Acid Recombination Interactions And Repair

DNA: Recombination, Interactions and Repair presents the result of the proceedings of the FEBS Symposium on DNA, which focuses on DNA recombination in vivo and in vitro, DNA interactions, and DNA repair. This book presents problems involving topics on DNA that have been studied intensively using methodological approaches and developed techniques for cloning, physical mapping, and sequencing of DNA. This text also notes that this approach has provided a basis for further genetic studies at the molecular level. The problems related to the genetic complexity of higher organisms and the relationships between the structure of genetic material and the regulation of expression of genetic information, as well as genetic engineering of micro-organisms, which become amenable to experimental investigation, are also presented in this text. This book notes that these problems are closely associated with endeavors to understand the molecular foundations of living processes to create a rational basis for treating genetic and viral diseases. This text will be a valuable source of information for students studying in the field of genetics, especially those conducting extensive research on DNA.

This book is a comprehensive review of the detailed molecular mechanisms of and functional crosstalk among the replication, recombination, and repair of DNA (collectively called the "3Rs") and the related processes, with special consciousness of their biological and clinical consequences. The 3Rs are fundamental molecular mechanisms for organisms to maintain and sometimes intentionally alter genetic information. DNA replication, recombination, and repair, individually, have been important subjects of molecular biology since its emergence, but we have recently become aware that the 3Rs are actually much more intimately related to one another than we used to realize. Furthermore, the 3R research fields have been growing even more interdisciplinary, with better understanding of molecular mechanisms underlying other important processes, such as chromosome structures and functions, cell cycle and checkpoints, transcriptional and epigenetic regulation, and so on. This book comprises 7 parts and 21 chapters: Part 1 (Chapters 1–3), DNA Replication; Part 2 (Chapters 4–6), DNA Recombination; Part 3 (Chapters 7–9), DNA Repair; Part 4 (Chapters 10–13), Genome Instability and Mutagenesis; Part 5 (Chapters 14–15), Chromosome Dynamics and Functions; Part 6 (Chapters 16–18), Cell Cycle and Checkpoints; Part 7 (Chapters 19–21), Interplay with Transcription and Epigenetic Regulation. This volume should attract the great interest of graduate students, postdoctoral fellows, and senior scientists in broad research fields of basic molecular biology, not only the core 3Rs, but also the various related fields (chromosome, cell cycle, transcription, epigenetics, and similar areas). Additionally, researchers in neurological sciences, developmental biology, immunology, evolutionary biology, and many other fields will find this book valuable.

"There are many forms of DNA damage. One of these, DNA-metal cross-links are particularly problematic as they not only affect the tautomeric structure of DNA, but can also inhibit enzymatic activity and block amplification with DNA polymerases. Since the inability to amplify DNA can affect many fields of genetic research, it is necessary to find ways to identify this damage and repair the DNA. Copper is one metal that can form metal-DNA cross-links and can seriously affect the recovery and analysis of degraded DNA from forensic or archaeological material. In this research DNA-copper cross-links were generated for the development of a repair method and applied to an archaeological sample. The DNA-copper adducts were identified through the use of gas chromatography-mass spectrometry on several templates of different complexity: single deoxyribonucleotides, a synthetic 22 base pair double stranded DNA fragment, modern amplified DNA, and an ancient extract with naturally occurring copper cross-links. A number of chemicals were considered for direct reversal repair of copper-DNA cross-links of which ethylenediamine was successful. Treatment of all the templates with ethylenediamine resulted in the repair of the nucleobase, specifically guanine which is the most susceptible to copper cross-link formation. The success of the direct reversal repair was verified using GC-MS based on expected retention time and the identification of ion fingerprints. The amount of copper-DNA adducts measured in each template varied greatly as did the success of the direct reversal repair although repair was evident in all samples."--Abstract.

This latest volume addresses the contemporary issues related to recombination in filamentous fungi, EST data mining, fungal intervening sequences, gene silencing, DNA damage response in filamentous fungi, cfp genes of Neurospora, developmental gene sequences, site-specific recombination, heterologous gene expression, hybridization and microarray technology to enumerate biomass. This volume also analyse the current knowledge in the area of hydrophobins and genetic regulation of carotenoid biosynthesis. Over fifty world renowned scientist from both industry and academics provided in-depth information in the field of fungal genes and genomics.