Cytometry 4th Edition

From the reviews of the 3rd Edition... "The standard reference for anyone interested in understandingflow cytometry technology." American Journal of Clinical Oncology "...one of the most valuable of its genre and...addressed to awide audience?written in such an attractive way, being bothinformative and stimulating." Trends in Cell Biology This reference explains the science and discusses the vastbiomedical applications of quantitative analytical cytology usinglaser-activated detection and cell sorting. Now in its fourthedition, this text has been expanded to provide full coverage ofthe broad spectrum of applications in molecular biology andbiotechnology today. New to this edition are chapters on automatedanalysis of array technologies, compensation, high-speed sorting,reporter molecules, and multiplex and apoptosis assays, along withfully updated and revised references and a list of suppliers.

Flow cytometry continually amazes scientists with its ever-expanding utility. Advances in flow cytometry have opened new directions in theoretical science, clinical diagnosis, and medical practice. The new edition of Flow Cytometry: First Principles provides a thorough update of this now classic text, reflecting innovations in the field while outlining the fundamental elements of instrumentation, sample preparation, and data analysis. Flow Cytometry: First Principles, Second Edition explains the basic principles of flow cytometry, surveying its primary scientific and clinical applications and highlighting state-of-the-art techniques at the frontiers of research. This edition contains extensive revisions of all chapters, including new discussions on fluorochrome and laser options for multicolor analysis, an additionalsection on apoptosis in the chapter on DNA, and new chapters onintracellular protein staining and cell sorting, including high-speed sorting and alternative sorting methods, as well as traditional technology. This essential resource: Assumes no prior knowledge of flow cytometry Progresses with an informal, engaging lecture style from simpleto more complex concepts Offers a clear introduction to new vocabulary, principles of instrumentation, and strategies for data analysis Emphasizes the theory relevant to all flow cytometry, with examples from a variety of clinical and scientific fields Flow Cytometry: First Principles, Second Edition provides scientists, clinicians, technologists, and students with the knowledge necessary for beginning the practice of flow cytometry and for understanding related literature.

The new edition promises a completely current overview of this increasingly commonplace technology. With every chapter either completely reworked or significantly updated to include the latest developments and new contributions from 10 new international authorities, this will be the go-to resource on clinical flow cytometry for years to come. More than that it promises to deliver a uniquely engaging learning experience with a bonus CD filled with real-world cases and interpretations. Drs. Carey and McCoy bring the new edition of 'Flow Cytometry in Clinical Diagnosis' alive with the bonus CD, which includes data from actual flow analysis and patient samples for 39 cases.

The chapters in CYTOMETRY MCB volumes, including this 4th Edition, provide comprehensive description of particular cytometric methods and review their applications. Some chapters also describe new instrumentation and provide fundamental information on use of new fluorescent probes and on data analysis. Although the term "edition" suggests the update of earlier volumes, in fact, nearly all chapters of the 4th Edition are devoted to new topics. The authors were invited to present not only technical protocols, such as available in other methodology books that specialize in the protocol format, but also to discuss the aspects of the methodology that generally are not included in the protocols. Many chapters, thus, present the theoretical foundations of the described methods, their applicability in experimental laboratory and clinical setting, common traps and pitfalls, problems with data interpretation, comparison with alternative assays, choice of the optimal assay, etc. Some chapters review applications of cytometry and complementary methodologies to particular biological problems or clinical tasks. Comprehensive presentation of cytometric methods covering theoretical applications, applicability, potential pitfalls, and comparisions to alternative assays Discusses many new assays developed since the previous edition Presents recent developments in cytometric intrumentation/technology

This book is a focused review of clinical flow cytometry, and is meant to be helpful in daily clinical practice for those just beginning to learn flow cytometry as well as those with years of experience. Covering the basic principles of flow cytometry, and then engaging in detailed reviews of the flow cytometric evaluation of B-cells, plasma cells, T-cells, and myeloid cells, it packs a wide variety of immunophenotypic data into one volume. Moreover, this book covers both normal and abnormal findings for each lineage, and highlights key pitfalls to avoid making diagnostic mistakes. Many of the most common neoplastic entities are reviewed, and signature findings are highlighted. Using the updated nomenclature for clinical hematologic malignancies provided by the revised 4th edition of the WHO classification system, the book is current in its approach and content. Whenever possible, detailed colored examples of flow cytometric plots are provided to help convey the important diagnostic findings. Most importantly, a review of current applications of flow cytometry in minimal residual disease is provided to assist in both the development and interpretation of these assays. Written by experts in the field, the result is a practical resource for use as an everyday clinical reference.

Platelets, Second Edition is the definitive current source of state-of-the-art knowledge about platelets and covers the entire field of platelet biology, pathophysiology, and clinical medicine. Recently there has been a rapid expansion of knowledge in both basic biology and the clinical approach to platelet-related diseases including thrombosis and hemorrhage. Novel platelet function tests, drugs, blood bank storage methods, and gene therapies have been incorporated into patient care or are in development. This book draws all this information into a single, comprehensive and authoritative resource. First edition won Best Book in Medical Science Award from the Association of American Publishers Contains fourteen new chapters on topics such as platelet genomics and proteomics, inhibition of platelet function by the endothelium, clinical tests of platelet function, real time in vivo imaging of platelets, and inherited thrombocytopenias A comprehensive full color reference comprising over 70 chapters, 1400 pages, and 16,000 references

Cytometry is characterization and measurement of cells and cellular constituents, most often used to immunophenotype cells - that is, to distinguish healthy cells from diseased cells. Flow Cytometry specifically is quite sensitive, allowing researchers to detect rare cell types and residual levels of disease, and as such has been the method of choice for important studies such as monitoring the blood of AIDS patients. For this reason, there is a great need for a practical, comprehensive manual that will be useful across a broad range of laboratories. This volume, as part of the Reliable Lab Solution Series, delivers such a tool, offering busy researchers across many disciplines a handy resource of all the best methods and protocols for Cytometry to use at the bench. Highlights top downloaded and cited chapters, authored by pioneers in the field and enhanced with their tips, and pitfalls to avoid Loaded with detailed protocols developed and used by leaders in the field Refines, organizes and updates popular methods from one of our top selling series, Methods in Cell Biology