Current Protocols In Toxicology Online

Toxicological research is driven by the need to understand and assess the human and ecological risks of exposure to chemicals and other toxicants as well as by interest in using toxic agents to elucidate basic biological and pathobiological processes. The level of research activity in this field is higher, the rate of change in knowledge more rapid, and interest in applying scientific information to societally important issues is greater than ever before. These are exciting and challenging times to be working in toxicology. The ongoing ferment builds on the extraordinary advances being made in the understanding of biological systems at the molecular level. This fundamental knowledge provides the opportunity for greatly enhanced insight into how chemicals and other stressors may damage biological structures and processes, influence the rate of biological repair, and lead to reversible or irreversible diseases or to a return to health. Society increasingly calls on the scientific community for the knowledge needed both to reevaluate the health hazards of existing products and technologies and to evaluate the prospective hazards of new ones. Such information is used to develop guidelines and regulations designed to ensure that these new products and technologies do not harm people or the environment. Acquiring sound, reproducible scientific data that can be integrated with existing information to advance the knowledge of toxicants and living systems requires rigorous adherence to the scientific method. This means intelligent, thoughtful individuals identifying important needs, formulating testable hypotheses, designing experiments to test them, meticulously conducting these experiments, carefully reviewing and interpreting it in the peer-reviewed literature. Current Protocols in Toxicology is a clear and well-documented compendium of the most important methods in the field?proven approaches developed by leading researchers?for the benefit of other experimentalists, from students to seasoned investigators. Since toxicology by its nature is multidisciplinary, other titles in the Current Protocols series may also provide relevant methods. Although review of the literature cited for each procedure can give added insight into the underlying theory and breadth of applications, the protocols have been carefully designed to provide clear, step-by-step descriptions that can easily be followed even by the relatively inexperienced.Regular updates to Current Protocols in Toxicology manual will help ensure an awareness of changes in previously documented methods and of methods newly developed. Use of these protocols will avoid unnecessary duplication of effort in development and validation when the methods are applied without modification, and will speed up the development of more refined methods that will further advance the field of toxicology and, in turn, may have a place in future updates.Roger O. McClellanChemical Industry of ToxicologyResearch Triangle Park, North Carolina

Current Protocols in Immunology is a three-volume looseleaf manual that provides comprehensive coverage of immunological methods from classic to the most cutting edge, including antibody detection and preparation, assays for functional activities of mouse and human cells involved in immune responses, assays for cytokines and their receptors, isolation and analysis of proteins and peptides, biochemistry of cell activation, molecular immunology, and animal models of autoimmune and inflammatory diseases. Carefully edited, step-by-step protocols replete with material lists, expert commentaries, and safety and troubleshooting tips ensure that you can duplicate the experimental results in your own laboratory. Bimonthly updates, which are filed into the looseleaf, keep the set current with the latest developments in immunology methods. The initial purchase includes one year of updates and then subscribers may renew their annual subscriptions. Current Protocols publishes a family of laboratory manuals for bioscientists, including Molecular Biology, Human Genetics, Protein Science, Cytometry, Cell Biology, Neuroscience, Pharmacology, and Toxicology.

The latest title from the acclaimed Current Protocols series, Current Protocols Essential Laboratory Techniques, 2e provides the new researcher with the skills and understanding of the fundamental laboratory procedures necessary to run successful experiments, solve problems, and become a productive member of the modern life science laboratory. From covering the basic skills such as measurement, preparation of reagents and use of basic instrumentation to the more advanced techniques such as blotting, chromatography and real-time PCR, this book will serve as a practical reference manual for any life science researcher. Written by a combination of distinguished investigators and outstanding faculty, Current Protocols Essential Laboratory Techniques, 2e is the cornerstone on which the beginning scientist can develop the skills for a successful research career.

Good methods must be reliable, well-tested, and honed to minimize the time and expense required to achieve the desired results. CPNC provides a continuously growing and evolving set of protocols that allows researchers to benefit from the experience of other researchers around the world. The core manual provides a comprehensive set of protocols that have been compiled, revised, and streamlined over the last 6 years. Quarterly updates provide new protocols in emerging areas of research as well as continued advances and new applications for fundamental methods. The book is designed to grow and change with the field of nucleic acid chemistry. Fundamental nucleoside chemistry methods include sugar-base condensation, phosphorylation, and nucleoside protection. Methods for oligonucleotide synthesis include H-phosphonate and phosphoramidite approaches, solid-phase and solution-phase synthesis, large-scale synthesis, synthesis for modified and unmodified oligonucleotides, conjugation of oligonucleotides, synthesis without base protection, and synthesis on microarrays. More specialized synthetic methods include synthesis of biologically active nucleosides and prodrugs. Purification and characterization methods are detailed. Advanced methods include biophysical analysis, combinatorial methods, and nanotechnology. Each protocol includes rationale for choosing appropriate methods, step-by-step procedures, complete recipes, anticipated results, characterization data, and troubleshooting, as well as background and recommended reading. The level of procedural detail is far beyond that found in the research literature, and tips and comments from authors are geared towards ensuring reliable duplication in the laboratory.

Provides the latest models and methods from molecular biology, cell biology, biochemistry, and genetics, plus sophisticated toxicological procedures from leading laboratories. Also offers guidelines for evaluating the effects of substances on human physiology and metabolism and reference information, including stock solutions and equipment and commonly used techniques.

Current Protocols in Bioinformatics is the only publication that responds to the need for both a current and updateable source of bioinformatics methodology. This unique publication assures that you have access to a full range of bioinformatics protocols written by globally-recognized experts in the field, and that these proto-cols are updated and revised as new developments and innovations occur.