Biotechnological Approaches For Genetic Improvement Of Sorghum

Sorghum (Sorghum bicolor L., Moench) is the fifth most important cereal crop in the world and represents an important source of food, feed and energy in several countries. Recently, there has been an increasing interest in sorghum cultivation worldwide, since it is relatively more drought- and heat-tolerant than other cereal crops, and it is better suited for the predicted consequences of global warming. In Africa and Asia, sorghum is primarily used as food for more than 500 million people, while in the Americas and Australia, it is used mainly as a maize-substitute in livestock feed. In the United States, sorghum is also being used in the production of ethanol. In view of its diverse utility, sorghum offers a large number of target traits that could be modified to meet the required applications. In this work, we have used different genetic engineering approaches to address two important issues in sorghum: seed quality and nitrogen use efficiency. First, we examined the temporal and spatial activity of a rice glutelin gene (GluA-2) promoter, in transgenic sorghum. Results from quantitative and histochemical GUS assays, as well as from transcript analyses, showed that this promoter is highly active during the middle stages of sorghum seed development and that it controls transgene expression specifically in the seed endosperm. This means that the GluA-2 promoter can serve as a useful tool in introducing novel traits into sorghum seed in order to improve the quality of this important cereal. Furthermore, we investigated the effects of cytosolic glutamine synthetase (GS1) and alanine aminotransferase (AlaAT) gene overexpression on nitrogen metabolism and plant growth in sorghum. T_(2) generation plants transformed with a sorghum GS1 gene (Gln1) driven by the maize ubiquitin promoter exhibited enhanced grain yield and biomass accumulation under optimal nitrogen levels. The electronic version of this dissertation is accessible from http://hdl.handle.net/1969.1/151264

This edited book is focused on Sustainable Development Goal 2, which aims to achieve 'Zero Hunger.' It provides deep insights into the global sorghum status, limitations to its production, advancements in agronomic practices, and the application of high-throughput phenotyping technologies. Sorghum plays a vital role in global food security, agricultural sustainability, and rural livelihoods, making it an important crop for both developing and developed countries. It is a staple food for millions of people around the world, particularly in arid and semi-arid regions where other crops may struggle to grow. Sorghum exhibits significant genetic diversity, providing a rich resource for breeding programs aimed at developing improved varieties with traits such as higher yield, disease resistance, and nutritional quality. The book enhances readers' understanding of classical breeding methods and their role in sorghum improvement. It also focuses on the contribution of OMICs and biotechnological approaches to sorghum improvement. Detailed information about the genetic and genomic resources of sorghum provided is helpful for the scientific community to utilize in sorghum breeding. Chapters highlight sorghum genome sequencing, transgenic and hybrid sorghum, and the application of genome editing. This book is useful to the breeding community, serving as a resource for interdisciplinary research groups such as geneticists, breeders, biotechnologists, bioinformaticians, and students, supporting them in accelerating their activities related to sorghum breeding.

This new volume, Advances in Sorghum Science: Botany, Production, and Crop Improvement, provides an easy-to-read and comprehensive treatment of the sorghum crop. With the world’s production of sorghum topping over 55 million tons annually, sorghum is very important for as a staple dietary food for much of the world as a rich source of micronutrients and macronutrients, as an ingredient in the processing of many foods, and as a source of fodder. The authors of the volume provide detailed information on sorghum from several disciplines and bring together recent literature under one umbrella. The book covers the various aspects of the sorghum crop, starting from its origin, to its domestication, and going on to biotechnology of the crop. It describes sorghum production, ideotypes, botany, physiology, abiotic and biotic factors affecting crop productivity, methods of cultivation, postharvest management, grain quality analysis for food processing, improvement of sorghum crop, and research advancements in breeding and biotechnology. This valuable resource will be helpful to researchers and scientists working to understand the relation between various disciplines and the implementation of new methods and technology for crop improvement and higher productivity. The multi-pronged approach will help to enable the increase sorghum productivity to meet the world’s growing demands.

Millets and sorghum are extremely important crops in many developing nations and because of the ability of many of them to thrive in low-moisture situations they represent some exciting opportunities for further development to address the continuing and increasing impact of global temperature increase on the sustainability of the world’s food crops. The main focus of this thorough new book is the potential for crop improvement through new and traditional methods, with the book’s main chapters covering the following crops: sorghum, pearl millet, finger millet, foxtail milet, proso millet, little millet, barnyard millet, kodo millet, tef and fonio. Further chapters cover pests and diseases, nutritional and industrial importance, novel tools for improvement, and seed systems in millets. Millets and Sorghum provides full and comprehensive coverage of these crucially important crops, their biology, world status and potential for improvement, and is an essential purchase for crop and plant scientists, and food scientists and technologists throughout the developed and developing world. All libraries in universities and research establishment where biological and agricultural sciences are studied and taught should have copies of this important book on their shelves.

Sorghum is one of the hardiest crop plants in modern agriculture and also one of the most versatile. Its seeds provide calorie for food and feed, stalks for building and industrial materials and its juice for syrup. This book provides an in-depth review of the cutting-edge knowledge in sorghum genetics and its applications in sorghum breeding. Each

During the past decade, there has been tremendous progress in maize biotechnology. This volume provides an overview of our current knowledge of maize molecular genetics, how it is being used to improve the crop, and future possibilities for crop enhancement. Several chapters deal with genetically engineered traits that are currently, or soon will be, in commercial production. Technical approaches for introducing novel genes into the maize genome, the regeneration of plants from transformed cells, and the creation of transgenic lines for field production are covered. Further, the authors describe how molecular genetic techniques are being used to identify genes and characterize their function, and how these procedures are utilized to develop elite maize germplasm. Moreover, molecular biology and physiological studies of corn as a basis for the improvement of its nutritional and food-making properties are included. Finally, the growing use of corn as biomass for energy production is discussed.

Nonrenewable energy resources deplete with the passage of time due to rapid increase in industrialization and population. Hence, countries worldwide are investing dearly in substitute energy resources like biofuel from miscellaneous set of feedstocks. Among the energy crops, sorghum serves as a model crop due to its drought tolerance, small genome size (730 Mb), high biomass, dry matter contents, quick growth, wide adaptability to diverse climatic and soil conditions and C4 photosynthesis. Sweet sorghum with high sugar content in stalk is an efficient feedstock for advanced biofuels and other bio-based products from sugars. However, high biomass sorghum has the utility as a feedstock for cellulosic biofuels. The enhanced yield of monomeric carbohydrates is a key to cheap and efficient biofuel production. The efficiency of lignocellulosic biofuels is compromised by recalcitrance to cell wall digestion, a trait that cannot be efficiently improved by traditional breeding. Therefore, scientists are looking for solutions to such problems in biomass crop genomes. Sorghum genome has been completely sequenced and hence this crop qualifies for functional genomics analysis by fast forward genetic approaches. This chapter documents the latest efforts on advancement of sorghum for biomass potential at morphological and molecular level by exploiting genomics approaches.

Focusing on the most important sorghum and millet diseases, this book is the result of an international conference on diseases of sorghum and millet from different growing regions of the world. New research approaches to improve disease management strategies are included.

This book covers a range of important topics on crop and animal genetics, breeding and genomics, as well as biodiversity and genetic resources conservation and utilization reflecting three thematic sections of working groups of the Biotechnology Society of Nigeria. The topics range from agricultural biotechnology, including genetically modified organisms and gene-editing for agronomically important traits in tropical crops, to Nigeria’s mega biodiversity and genetic resources conservation. This book will engender a deeper understanding of underpinning mechanisms, technologies, processes and science–policy nexus that has placed Nigeria as a leader in biotechnology in Africa. The book will be useful reference material for scientists and researchers working in the fields of food and agricultural biotechnology, bioinformatics, plant and animal genetics, breeding and genomics, genetic resources conservation and enhancement. Emphasizes recent advances in biotechnologies that could ameliorate the high-level global food and nutrition insecurity through plant and animal genetics, breeding, as well as genomics Provides detailed information towards harnessing indigenous bioresources for food and nutrition security and climate change adaptation Introduces new frontiers in the area of genomics, most especially their relevant applications in crop and animal breeding Reviews biotechniques that could enhance plant genetic resources conservation and utilization Discusses current biotechnological approaches to exploit genetic resources including the development of synthetic hexaploid wheat (SHW) for crop adaptation to the increasingly changing global climate