Applications Of Omics In Plant Microbiome Interactions

This book presents a timely review of the latest advances in rhizosphere biology, which have been facilitated by the application of omics tools. It includes chapters on the use of various omics tools in rhizosphere biology, focusing on understanding plant and soil microbe interactions. The role of proteomics and metagenomics in research on symbiotic association is also discussed in detail. The book also includes chapters on the use of omics tools for the isolation of functional biomolecules from rhizospheric microorganisms. The book’s respective sections describe and provide detailed information on important omics tools, such as genomics, transcriptomics, proteomics, metabolomics and meta-epigenomics. In turn, the book promotes and describes the combined use of plant biology, microbial ecology, and soil sciences to design new research strategies and innovative methods in soil biology. Lastly, it highlights the considerable potential of the rhizosphere in terms of crop productivity, bioremediation, ecological engineering, plant nutrition and health, as well as plant adaptation to stress conditions. This book offers both a practical guide and reference source for all scientists working in soil biology, plant pathology, etc. It will also benefit students studying soil microbiology, and researchers studying rhizosphere structure.

Microbiomics and Sustainable Crop Production Microbiomics and Sustainable Crop Production presents an overview of the current state of the art in microbiome research, discussing many new technologies and approaches in order to bridge knowledge gaps between field and lab experimental systems. New and emerging strategies to improve the survival and activity of microbial inoculants are covered, including the use of selected indigenous microbes, optimizing microbial delivery methods, and taking advantage of modern gene editing tools to engineer microbial inoculants. The two highly qualified authors address new molecular tools and powerful biotechnological advances, providing readers with knowledge of the complex chemical and biological interactions that occur in the rhizosphere and ensuring that strategies to engineer the rhizosphere are safe, beneficial to productivity, and result in improvements to the sustainability of agricultural systems. The relationship between phyllosphere microbial communities and functional traits of plants is also explored. Finally, approaches and priority areas for future research on phyllosphere microbiology are suggested. Topics covered in this comprehensive resource include: Transmission modes of bacteria and fungi and the nature of their interactions in the endosphere Characteristics of ‘core microbiomes’, which may be deployed to organize otherwise uncontrollable dynamics of resident microbiomes Model microbiome-plant systems, as well as the stability, resilience, and assembly of agricultural microbiomes Engineering and management of agricultural microbiomes for improving crop health, including reasons to modify plant microbiomes Microbiome research in the omics era and new efforts and challenges in assigning functions to microbes For students of plant biotechnology, agricultural sciences, and agricultural engineering, along with researchers working in related fields, Microbiomics and Sustainable Crop Production is an important resource to understand many complex modern ideas related to the subject and how they can be applied to practical applications.

Key Features: Shares the latest insight on omics technologies to unravel plant-microbe dynamic interactions and other novel phytotechnologies for cleaning contaminated soils. It also provides brief insight on the recently discovered clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9-a genome editing tool to explore plant-microbe interactions and how this genome editing tool helps to improve the ability of microbes/plants to combat abiotic/biotic stresses.

This book is a concerted effort to put together the rapidly growing facets of biological data. It provides a platform for the readers to think about integrative approaches to solve complex biological problems. This fundamental book deals with the simplest concepts of omics to recent advancements in the field. The content is divided into seven chapters that provide insight into various omics approaches, omics technologies, and its applications. Each chapter delves into different molecular scales: genomics, transcriptomics, proteomics, and metabolomics. Further to provide a holistic view a chapter detailing microbiome has been included in the book. The sub-sections in the chapters is dedicated to introducing the various analytical tools such as next generation sequencing, chromatin immunoprecipitation, mass spectrometry, peptide mass fingerprinting, RNA Seq and NMR spectroscopy. It entails a chapter focused on the bioinformatics resources for analysis of the omics data. In summary, this comprehensive book emphasizes the recent advancements in the study of biomolecules spanning from DNA to metabolites.

A constant research effort to understand plant-microbe interactions makes it indispensable to keep abreast of the latest research developments. Researchers from a range of disciplines have used multiple approaches to infer this field. With the advent of next-generation techniques, both molecular and computational, the field has entered a new phase. These approaches often result in massive information, which is sometimes tangled and in need of further analysis. These types of analyses also require cutting-edge data analytics as well as efficient statistical models. Plant-Microbe Interactions: Harnessing Next-Generation Molecular Technologies for Sustainable Agriculture provides a comprehensive picture of the modern-day analytics and approaches being used to provide insights into the interactions between plant and microbe. A wide range of technologies are explored along with practical guides toward these techniques. A detailed understanding of omics data in various areas could be obtained from this compilation. Key Features: • Crosstalk between plant and microbe • Overview of advanced molecular techniques used to study plant-microbe interaction • Practical guide to technologies such as NGS • Omics data analysis used to study plant-microbe interaction • Role of soil metagenomics • Advanced technologies such as nanotechnology and CRISPR serving to study plant-microbe interaction This book will serve as a great reference to various next-generation techniques in the field of plant-microbe interaction, thereby helping to better understand the mechanism. This will also help budding researchers to shape their research in similar areas.

Technologies collectively called omics enable simultaneous measurement of an enormous number of biomolecules; for example, genomics investigates thousands of DNA sequences, and proteomics examines large numbers of proteins. Scientists are using these technologies to develop innovative tests to detect disease and to predict a patient's likelihood of responding to specific drugs. Following a recent case involving premature use of omics-based tests in cancer clinical trials at Duke University, the NCI requested that the IOM establish a committee to recommend ways to strengthen omics-based test development and evaluation. This report identifies best practices to enhance development, evaluation, and translation of omics-based tests while simultaneously reinforcing steps to ensure that these tests are appropriately assessed for scientific validity before they are used to guide patient treatment in clinical trials.

Beneficial Plant-microbial Interactions: Ecology and Applications provides insight into the mechanisms underlying the interactions of plants and microbes, the ecological relevance and roles of these symbioses, the adaptive mechanisms of plant-associated microorganisms to abiotic stress and their contribution to plant stress tolerance, and the poten

Plants are typically colonized by numerous endophyte species symbiotically without any noticeable disease symptoms. These microbes are abundant, diverse and play critical ecological roles across natural and agricultural ecosystems. Endophytes have attracted the attention of researchers due to their various beneficial effects on plants, especially in agricultural crop species. Genomic tools will enhance our understanding on the growth and nutrition requirements of this host-symbiont relationship. Recent advances in DNA sequencing technologies and bioinformatic pipelines have allowed analyzing the plant microbiome and host-endophyte interaction more effectively with limited bias. Furthermore, various studies have employed and utilized transcriptomic and genomic tools to understand the role of endophytes and their interaction with plant hosts. This electronic book covers various research articles highlighting the important developments on endophytes using transcriptomics, next generation sequencing and genomic tools.

The study of plant-microbe associations by new techniques has significantly improved our understanding of the structure and specificity of the plant microbiome. Yet, microbiome function and the importance of the plant’s microbiome in the context of human and plant health are largely unexplored. Comparable with our human microbiome, millions of microbes inhabit plants, forming complex ecological communities that influence plant growth and health through its collective metabolic activities and host interactions. Viewing the microbiota from an ecological perspective can provide insight into how to promote plant health and stress tolerance of their hosts or how to adapt to a changing climate by targeting this microbial community. Moreover, the plant microbiome has a substantial impact on human health by influencing our gut microbiome by eating raw plants such as lettuce and herbs but also by influencing the microbiome of our environment through airflow. This research topic comprising reviews, original and opinion articles highlights the current knowledge regarding plant microbiomes, their specificity, diversity and function as well as all aspects studying the management of plant microbiomes to enhance plant growth, health quality and stress tolerance.