Analysis Of Enzyme Kinetic Data
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Author | : Athel Cornish-Bowden |
Publisher | : Oxford University Press, USA |
Total Pages | : 224 |
Release | : 1995 |
Genre | : Medical |
ISBN | : |
Download Analysis of Enzyme Kinetic Data Book in PDF, Epub and Kindle
This book introduces the theory and practice of statistical analysis of kinetic data for enzyme-catalysed reactions in the steady state. It includes a detailed description of the use of the Leonora program for analysing enzyme kinetic data, together with the program on an IBM PC compatibledisk. Default options and a worked example provide invaluable guidance for the student and the novice and in addition Analysis of Enzyme Kinetic Data provides each reader with the necessary software and the required understanding to tailor an analysis to the requirements of their own research. Theoretical topics include basic principles of a least squares analysis; fitting the Michaelis-Menten equation by a least squares analysis; the general linear model; residual plots; maximum likdlihood and efficiency; generalised medians; and robust regression. Practical topics include examinationand fitting of statistical data; installation of Leonora, its use, simulations, MENUs, and customization.
Author | : Laszlo Endrenyi |
Publisher | : Springer Science & Business Media |
Total Pages | : 420 |
Release | : 2012-12-06 |
Genre | : Technology & Engineering |
ISBN | : 1461332559 |
Download Kinetic Data Analysis Book in PDF, Epub and Kindle
Kinetic models have often served as useful examples in develop ing the methodology for the design and analysis of experiments in volving mechanistic models. Thus, it is not surprising that these approaches have been applied quite successfully to kinetic obser vations. Nevertheless, many ideas and methods were developed indepen dently in various fields of science. More often than not, investi gators working in one area have not been aware of relevant advances in others. In order to facilitate the desirable exchange of ideas, a one-day symposium was held in Toronto in conjunction with the XIth International Congress of Biochemistry. Biochemists, pharmacolo gists,> and statisticians came together and discussed many of the topics presented in this volume. Participants in the symposium believed that it would be use ful to publish a collection of the presentations together with some additional material. The present volume is the result. It is an attempt to convey some of the interdisciplinary concerns involv ing mechanistic, and especially kinetic, model building. The coverage is by no means exhaustive: many principles, methods, and problems are not included. Even the applications are limited to biochemistry and pharmacology. Still, the symposium highlighted areas of current interest. These included questions of weighting, robust parameter estimation, pooled data analysis, model identification, and the design of experiments. These topics, which are of interest in many fields of science, are discus3ed also in the present volume.
Author | : N.S. Punekar |
Publisher | : Springer |
Total Pages | : 562 |
Release | : 2018-11-11 |
Genre | : Science |
ISBN | : 9811307857 |
Download ENZYMES: Catalysis, Kinetics and Mechanisms Book in PDF, Epub and Kindle
This enzymology textbook for graduate and advanced undergraduate students covers the syllabi of most universities where this subject is regularly taught. It focuses on the synchrony between the two broad mechanistic facets of enzymology: the chemical and the kinetic, and also highlights the synergy between enzyme structure and mechanism. Designed for self-study, it explains how to plan enzyme experiments and subsequently analyze the data collected. The book is divided into five major sections: 1] Introduction to enzymes, 2] Practical aspects, 3] Kinetic Mechanisms, 4] Chemical Mechanisms, and 5] Enzymology Frontiers. Individual concepts are treated as stand-alone chapters; readers can explore any single concept with minimal cross-referencing to the rest of the book. Further, complex approaches requiring specialized techniques and involved experimentation (beyond the reach of an average laboratory) are covered in theory with suitable references to guide readers. The book provides students, researchers and academics in the broad area of biology with a sound theoretical and practical knowledge of enzymes. It also caters to those who do not have a practicing enzymologist to teach them the subject.
Author | : Alejandro G. Marangoni |
Publisher | : John Wiley & Sons |
Total Pages | : 247 |
Release | : 2003-04-23 |
Genre | : Science |
ISBN | : 0471461415 |
Download Enzyme Kinetics Book in PDF, Epub and Kindle
Practical Enzyme Kinetics provides a practical how-to guide for beginning students, technicians, and non-specialists for evaluating enzyme kinetics using common software packages to perform easy enzymatic analyses.
Author | : Ron KENETT |
Publisher | : |
Total Pages | : |
Release | : 1979 |
Genre | : |
ISBN | : |
Download STATISTICAL ANALYSIS OF ENZYME KINETIC DATA Book in PDF, Epub and Kindle
Author | : Athel Cornish-Bowden |
Publisher | : Elsevier |
Total Pages | : 245 |
Release | : 2014-05-20 |
Genre | : Science |
ISBN | : 1483161196 |
Download Fundamentals of Enzyme Kinetics Book in PDF, Epub and Kindle
Fundamentals of Enzyme Kinetics details the rate of reactions catalyzed by different enzymes and the effects of varying the conditions on them. The book includes the basic principles of chemical kinetics, especially the order of a reaction and its rate constraints. The text also gives an introduction to enzyme kinetics - the idea of an enzyme-substrate complex; the Michaelis-Menten equation; the steady state treatment; and the validity of its assumption. Practical considerations, the derivation of steady-state rate equations, inhibitors and activators, and two-substrate reactions are also explained. Problems after the end of each chapter have also been added, as well as their solutions at the end of the book, to test the readers' learning. The text is highly recommended for undergraduate students in biochemistry who wish to study about enzymes or focus completely on enzymology, as most of the mathematics used in this book, which have been explained in detail to remove most barriers of understanding, is elementary.
Author | : Daniel L. Purich |
Publisher | : Elsevier |
Total Pages | : 915 |
Release | : 2010-06-16 |
Genre | : Science |
ISBN | : 0123809258 |
Download Enzyme Kinetics: Catalysis and Control Book in PDF, Epub and Kindle
Far more than a comprehensive treatise on initial-rate and fast-reaction kinetics, this one-of-a-kind desk reference places enzyme science in the fuller context of the organic, inorganic, and physical chemical processes occurring within enzyme active sites. Drawing on 2600 references, Enzyme Kinetics: Catalysis & Control develops all the kinetic tools needed to define enzyme catalysis, spanning the entire spectrum (from the basics of chemical kinetics and practical advice on rate measurement, to the very latest work on single-molecule kinetics and mechanoenzyme force generation), while also focusing on the persuasive power of kinetic isotope effects, the design of high-potency drugs, and the behavior of regulatory enzymes. Historical analysis of kinetic principles including advanced enzyme science Provides both theoretical and practical measurements tools Coverage of single molecular kinetics Examination of force generation mechanisms Discussion of organic and inorganic enzyme reactions
Author | : Peter J. F. Henderson |
Publisher | : |
Total Pages | : 43 |
Release | : 1978 |
Genre | : Enzyme kinetics |
ISBN | : 9780444800626 |
Download Statistical Analysis of Enzyme Kinetic Data Book in PDF, Epub and Kindle
Author | : H. J. Fromm |
Publisher | : Springer Science & Business Media |
Total Pages | : 332 |
Release | : 2013-11-11 |
Genre | : Science |
ISBN | : 3642809669 |
Download Initial Rate Enzyme Kinetics Book in PDF, Epub and Kindle
Enzyme kinetics has undergone very rapid growth and development during the past fifteen years and has been well received by the biochemical community. A cursory glance at the current biochem ical literature reveals the increasing popularity of enzyme ki netics1 yet, there are very few books available to guide the enzymologist who wishes to conduct kinetic experiments. This monograph was undertaken to provide the fledgling kineticist with an outline of contemporary initial rate enzyme kinetics. A large portion of the material contained in this book is presented in a second-year, graduate-level course in biochemistry at Iowa State University. I have found that the presentation in this course has enabled students without a strong background in math ematics to undertake initial rate studies at the research bench. The monograph obviously is more comprehensive than any course could be, and should permit similar accomplishment. As the title implies, the major emphasis of this monograph is on initial rate enzyme kinetics. I considered at length the advis ability of including chapters on integrated rate equations and on the theory and application of rapid reaction kinetics, such as rapid-mixing stopped-flow, and temperature-jump kinetics. These, however, are topics that would require a good deal of space to develop if they were to be helpful to the beginner.
Author | : Irwin H. Segel |
Publisher | : |
Total Pages | : 957 |
Release | : 2014 |
Genre | : Enzyme kinetics |
ISBN | : 9788126548156 |
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